Forum
Human Cloning Foundation Hails British Scientists
Paralyzed
Walk Again
Childless Couples
Essays
The Benefits of
Human Cloning
All the Reasons to
Clone Human Beings
The Top Ten Myths
about Human Cloning
Human Cloning is the Cure for Infertility
Infertility is
a Disease
Books
People
Reports
Archives
Feedback
Donate
Links
Website Links
About Us
Contact Us
Site history
Site Map
Past Books of the Month
John Kunich's Books
Copyright
Archives New to Old
Archives Old to New
Illegal Beings: Human Clones
Cytogenetic analysis of diploidy in cloned bovine embryos using an improved air-dry karyotyping m
Li GP, Liu Y, White KL, Bunch TDTheriogenology. 2005 Jun;63(9):2434-44. Epub 2004 Dec 9..
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USA.
Of the few published studies on the cytogenetic analyses of bovine nuclear transferred (NT) embryos, results differ between air-dry and fluorescent in situ hybridization (FISH) procedures. A modified air-dry procedure is reported in this study that provides more metaphase plates for analysis. Day 5 and Day 7 bovine NT embryos were cultured in colcemid-containing CR1aa for 10-12 or 16-18 h, then treated in hypotonic sodium citrate for 3-5 min. The standard procedure of 5h in colcemid and 15-20 min in hypotonic solution was the control. A much higher (P<0.01) percent of mitotic nuclei was observed in the experimental groups. The 33 and 41% mitotic nuclei were obtained from 10 to 12 h and 16 to 18 h-colcemid-treated Day 5 embryos, respectively, which was higher (P<0.001) than the control (15%). The mitotic nuclei in Day 7 NT embryos were 24% in 10-12 h- and 28% in 16-18 h-colcemid-treated groups, which also was higher (P<0.05) than the control (10%). The majority of analyzable embryos were diploid. Analyses of mixoploid embryos showed on average that 70% of the cells were diploid. Day 5 mixoploid embryos contained numerically higher polyploid cells than Day 7 embryos, although statistically there were no differences. We concluded that the modified air-dry method provided a larger source of mitotic nuclei for chromosome analyses of cloned bovine embryos.
This Message is being posted for educational purposes, as well as for comment and criticism, by the visitors to the HumanCloning.org Foundation website (http://www.humancloning.org ).
Disclaimer: This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Human Cloning Foundation website (www.HumanCloning.org ). This abstract is representative of a larger article that is indexed on Medline.
The Human Cloning Foundation was established February, 1988. .
Who's Afraid of Human Cloning?
Disease Prevention and Treatment