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Growth and fertility of bulls cloned from the somatic cells of an aged and infertileShiga K, Umeki H, Shimura H, Fujita T, Watanabe S, Nagai TTheriogenology. 2005 Jul 15;64(2):334-43. Epub 2005 Jan 23.. Oita Prefectural Institute of Animal Industry, Kuju Naoiri, Oita 878-0201, Japan. In the present study, somatic cell cloning technology was used to produce eight newborn calves from an aged, infertile bull. Average birth weight of these calves was significantly higher than that of calves produced using AI. Four of the cloned calves died during the peripartum period; the remaining four (Clones A-D) survived and were used in this study. Two of the surviving calves (Clones C and D) were castrated; growth rates of the intact and castrated clones were similar to those of intact and castrated bulls, respectively, that had been derived by AI. Both uncastrated bulls (Clones A and B) began to produce normal semen at approximately 12 months of age. Semen produced by these clones, and their nuclear donor, was subsequently used for IVF; the proportion of IVM-IVF oocytes developing to the blastocyst stage was 23.4% (50/214), 28.4% (52/183) and 30.9% (63/204), respectively. Conception rates for AI were 54.5% (12/22) and 62.7% (64/102) for semen derived from Clone A and from the nuclear donor, respectively. The length of pregnancy and birth weight of the calves derived from semen collected from clones were similar to those of calves obtained by conventional AI using semen from their nuclear donor. Therefore, sires cloned from the somatic cells of an aged and infertile bull had normal fertility. ![]() This Message is being posted for educational purposes, as well as for comment and criticism, by the visitors to the HumanCloning.org Foundation website (http://www.humancloning.org ). Disclaimer: This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Human Cloning Foundation website (www.HumanCloning.org ). This abstract is representative of a larger article that is indexed on Medline. The Human Cloning Foundation was established February, 1988. . |
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