Forum
Human Cloning Foundation Hails British Scientists
Paralyzed
Walk Again
Childless Couples
Essays
The Benefits of
Human Cloning
All the Reasons to
Clone Human Beings
The Top Ten Myths
about Human Cloning
Human Cloning is the Cure for Infertility
Infertility is
a Disease
Books
People
Reports
Archives
Feedback
Donate
Links
Website Links
About Us
Contact Us
Site history
Site Map
Past Books of the Month
John Kunich's Books
Copyright
Archives New to Old
Archives Old to New
Illegal Beings: Human Clones
Mouse embryos and chimera cloned from neural cells in the postnatal cerebral c
Makino H, Yamazaki Y, Hirabayashi T, Kaneko R, Hamada S, Kawamura Y, Osada T, Yanagimachi R, Yagi TCloning Stem Cells. 2005;7(1):45-61..
Department of Physiological Sciences, Graduate University for Advanced Studies, Osaka, Japan.
Cloning of mice has been achieved by transferring nuclei of various types of somatic cell nuclei into enucleated oocytes. However, all attempts to produce live cloned offspring using the nuclei of neurons from adult cerebral cortex have failed. Previously we obtained cloned mice using the nuclei of neural cells collected from fetal cerebral cortex. Here, we attempted to generate cloned mice using differentiated neurons from the cerebral cortex of postnatal (day 0-4) mice. Although we were unable to obtain live cloned pups, many fetuses reached day 10.5 days of development. These fetuses showed various abnormalities such as spherical omission of the neuroepithelium, collapsed lumen of neural tube, and aberrant expressions of marker proteins of neurons. We produced chimeric mice in which some hair cells and kidney cells were originated from differentiated neurons. In chimeric fetuses, LacZ-positive donor cells were in all three germ cell layers. However, chimeras with large contribution of donor-derived cells were not obtained. These results indicate that nuclei of differentiated neurons have lost their developmental totipotency. In other words, the conventional nuclear transfer technique does not allow nuclei of differentiated neurons to undergo complete genomic reprogramming required for normal embryonic development.
This Message is being posted for educational purposes, as well as for comment and criticism, by the visitors to the HumanCloning.org Foundation website (http://www.humancloning.org ).
Disclaimer: This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Human Cloning Foundation website (www.HumanCloning.org ). This abstract is representative of a larger article that is indexed on Medline.
The Human Cloning Foundation was established February, 1988. .
Who's Afraid of Human Cloning?
Disease Prevention and Treatment