Forum
Human Cloning Foundation Hails British Scientists
Paralyzed
Walk Again
Childless Couples
Essays
The Benefits of
Human Cloning
All the Reasons to
Clone Human Beings
The Top Ten Myths
about Human Cloning
Human Cloning is the Cure for Infertility
Infertility is
a Disease
Books
People
Reports
Archives
Feedback
Donate
Links
Website Links
About Us
Contact Us
Site history
Site Map
Past Books of the Month
John Kunich's Books
Copyright
Archives New to Old
Archives Old to New
Illegal Beings: Human Clones
Identification of a new gene product (diphor-1) regulated by dietary phos
Custer M, Spindler B, Verrey F, Murer H, Biber JAm J Physiol. 1997 Nov;273(5 Pt 2):F801-6..
Institute of Physiology, University of Zurich, Switzerland.
Chronic restriction of dietary Pi elicits an increased reabsorption of Pi in the kidney proximal tubules, which involves a stimulation of apical Na-Pi cotransport. This adaptation is in part a direct cellular response of which the mechanism(s) are poorly understood. In this study, the impact of dietary Pi restriction on the differential expression of rat kidney cortex mRNAs was visualized to identify gene products regulated by the Pi status. When kidney cortex mRNAs of rats fed a low- or a high-Pi diet were compared by differential display-polymerase chain reaction (DD-PCR), thirty modulated cDNA bands were observed, of which four were confirmed as being regulated. We focused on one of the upregulated bands, dietary Pi-regulated RNA-1 (diphor-1). A cDNA containing an open reading frame encoding a 52-kDa protein was cloned by library screening. Diphor-1 exhibits a high degree of identity to the Na/H exchanger regulatory factor and to a tyrosine kinase activating protein. Highest expression of diphor-1 mRNA was detected in the kidney (proximal tubules) and in small intestine. Expression experiments showed that diphor-1 specifically increases Na-Pi cotransport in oocytes of Xenopus laevis coinjected with renal type II Na-Pi contransporter cRNA. Further characterizations of diphor-1 will show whether diphor-1 is primarily or secondarily involved in the response to dietary Pi.
This Message is being posted for educational purposes, as well as for comment and criticism, by the visitors to the HumanCloning.org Foundation website (http://www.humancloning.org ).
Disclaimer: This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Human Cloning Foundation website (www.HumanCloning.org ). This abstract is representative of a larger article that is indexed on Medline.
The Human Cloning Foundation was established February, 1988. .
Who's Afraid of Human Cloning?
Disease Prevention and Treatment