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Illegal Beings: Human Clones
Cryopreservation of plumular explants of coconut (Cocos nucifera L.) to support programmes for mass clonal propagation through somatic embryoge
Hornung R, Domas R, Lynch PTCryo Letters. 2001 Jul-Aug;22(4):211-20..
Plant Biotechnology Laboratories, Imperial College at Wye, T.H. Huxley School, Wye, Ashford, Kent, TN25 5AH, UK.
Callus growth has been observed from plumules of ecotype Laguna Tall after cryopreservation using an encapsulation/dehydration protocol. Sucrose preculture treatment and silica gel dehydration both significantly influenced the frequency of callus formation from non-frozen and frozen plumules. The greatest frequency of post-thaw callus growth occurred after incubation of the encapsulated plumules for 72-96 h in medium containing 0.75 M sucrose followed by desiccation over silica gel for 7-8 h down to approximately 30% moisture content (fresh weight basis). Freezing and thawing were carried out rapidly. Post-thaw recovery rates in excess of 80% were recorded.
This Message is being posted for educational purposes, as well as for comment and criticism, by the visitors to the HumanCloning.org Foundation website (http://www.humancloning.org ).
Disclaimer: This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Human Cloning Foundation website (www.HumanCloning.org ). This abstract is representative of a larger article that is indexed on Medline.
The Human Cloning Foundation was established February, 1988. .
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